Author Topic: TUNEL staining in osteoblast  (Read 3056 times)

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Offline LFong

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TUNEL staining in osteoblast
« on: February 28, 2012, 05:33:58 AM »
Dear all,

i perform a TUNEL staining on young canine bone (formalin fixed and decalcified in EDTA) and it stained the chondrocytes perfectly. However, i did see some cytoplasmic staining at the osteoblast (just beneath the calcifying zone), but not the nuclei. i repeated it twice and same findings. Is it due to aspecific staining?

Thank you for your advice!

LFong

TUNEL staining in osteoblast
« on: February 28, 2012, 05:33:58 AM »