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Author Topic: does triton or twee reduce membrane protein stain intensity in paraffin secton  (Read 799 times)

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Offline FMaxiao

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does triton or twee reduce membrane protein stain intensity in paraffin secton
« on: December 30, 2011, 11:57:03 PM »
I am doing the LGR5 protein detection in mouse intestine(paraffin based sectin,3um thick). LGR5 is a membrane G protein coupled receptor. Does triton penetration treatment induce the LGR5 intensity? any help will great appreciated.
Yours
Xiaofei Ma
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does triton or twee reduce membrane protein stain intensity in paraffin secton
« on: December 30, 2011, 11:57:03 PM »

Offline DCundiff

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Re: does triton or twee reduce membrane protein stain intensity in paraffin secton
« Reply #1 on: March 05, 2012, 02:56:24 PM »
According to an AbCam technical bulletin, Triton is the most popular detergent for   improving the penetration of an antibody, however, it is not appropriate for the use of membrane associated antigens since it destroys the membranes.  Hope this helps.
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Re: does triton or twee reduce membrane protein stain intensity in paraffin secton
« Reply #1 on: March 05, 2012, 02:56:24 PM »
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