Abcam Ad

Author Topic: Detection of neutrophils in mouse lungs  (Read 3501 times)

0 Members and 1 Guest are viewing this topic.

Offline PGamradt

  • Newbie
  • *
  • Posts: 1
Detection of neutrophils in mouse lungs
« on: June 05, 2012, 05:43:53 AM »
Hello,

I'm searching for a method to detect neutrophils in paraffin sections from mouse lungs.
As it is also recommended for paraffin sections, used the Naphtol AS-D Chloroacetate from Sigma-Aldrich.
However, I was not very successful detected  the neutrophils even though I could clear see them  in the H&E staining. Unfortunately this is not good enough for my purpose.
To be sure the kit worked I also stained some liver sections with huge infiltrates and here I was able to see the neutrophils appearing in light pink as expected.

Is there something special about lung tissue that I have to consider? Or are there other good methods to detect such cells in the lung?

I would be very happy if you could make some suggestions on my problem!
Thank you in advance!
pia

Detection of neutrophils in mouse lungs
« on: June 05, 2012, 05:43:53 AM »

Offline gula

  • Global Moderator
  • GoldMember
  • *****
  • Posts: 356
Re: Detection of neutrophils in mouse lungs
« Reply #1 on: June 05, 2012, 10:39:51 AM »
Try a PAS-stain.
gula

Re: Detection of neutrophils in mouse lungs
« Reply #1 on: June 05, 2012, 10:39:51 AM »