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Detection of neutrophils in mouse lungs

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PGamradt:
Hello,

I'm searching for a method to detect neutrophils in paraffin sections from mouse lungs.
As it is also recommended for paraffin sections, used the Naphtol AS-D Chloroacetate from Sigma-Aldrich.
However, I was not very successful detected  the neutrophils even though I could clear see them  in the H&E staining. Unfortunately this is not good enough for my purpose.
To be sure the kit worked I also stained some liver sections with huge infiltrates and here I was able to see the neutrophils appearing in light pink as expected.

Is there something special about lung tissue that I have to consider? Or are there other good methods to detect such cells in the lung?

I would be very happy if you could make some suggestions on my problem!
Thank you in advance!
pia

gula:
Try a PAS-stain.
gula

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