Author Topic: Ladder Ghost Bands  (Read 3281 times)

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Offline MGoodfellow

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Ladder Ghost Bands
« on: June 27, 2012, 11:47:32 AM »
I have been unable to get real bands for my ladder- just ghost bands. I use 10 ul (the recommended volume) in 4-20% mini gels and transfer for 1 hr at 100V. I have tried decreasing my secondary concentration, increasing blocking time/concentration, and decreasing ECL substrate incubation time- still no ladder. What else can I try? I'm new to WB so any suggestions would be appreciated.

Ladder Ghost Bands
« on: June 27, 2012, 11:47:32 AM »

Offline Sindy

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  • Posts: 9
Re: Ladder Ghost Bands
« Reply #1 on: January 17, 2013, 12:11:34 AM »
 :)Hi there. I am not sure.. are you using a strep tag ladder ?

Re: Ladder Ghost Bands
« Reply #1 on: January 17, 2013, 12:11:34 AM »