Author Topic: Concentrate the proteins or precipitate them?  (Read 2800 times)

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Offline MMehdi

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Concentrate the proteins or precipitate them?
« on: July 12, 2012, 11:05:15 AM »
Hi,

Either due to an error in the Bradford or too much dilution with water, my diluted samples (with sample buffer) of retinas contain a variable amount of protein, checked by beta-actin. The maximum sample size is 700 micro liters. I want to re-assay proteins in my samples. Bradford seems to be incompatible with Lamelli-sample buffer detergents. I'm looking for a protein assay which is compatible with sample buffer and other salts of Protein extraction buffer. Please do not suggest commercial kits.
Thanks

Concentrate the proteins or precipitate them?
« on: July 12, 2012, 11:05:15 AM »