I usually fix my ligament and tendon samples for 24 hours in zinc formalin. They are 1cm X 1cm tissues and they are thick. They are coronal longitudinal sections of ACL and tendon. After fixing them they are sent to Jefferson University where they are infiltrated. The Infiltration details are:1 hour each: 2x 70% Et-OH, 2x 95% Et-OH, 2x 100% Et-OH, 2 hours each: 2x Xylene ,4x paraffin All under vacuum(as per Dr. Freeman) After infiltration I embed them. After taking some sections so that the tissue in the block is exposed to the air, I soak the whole block in downy for about 45-50 minutes and I section them. The sections were not of uniform thickness also they crumble apart when I section. Once I socked them for longer duration for example 1and half hour , the sections were better, but I am not sure if I should sock them this long. The tissue was swollen and was protruding from the paraffin blocks.
Could you please give me some helpful tips/advise?