Author Topic: Cell culture maintenance and confluency  (Read 3325 times)

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Offline ic2133

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Cell culture maintenance and confluency
« on: December 17, 2012, 12:02:26 PM »
Hi,

I am growing HEK 293 cells. I know you're supposed to passage them at 70%, but I let them grow to 100% by accident.  Can I still pass them and maintain them, as long as my experiments are done on 70% confluent cells? How bad is it that it got to 100%?

Thank you!

Cell culture maintenance and confluency
« on: December 17, 2012, 12:02:26 PM »

Offline AArwen

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Re: Cell culture maintenance and confluency
« Reply #1 on: January 16, 2013, 05:43:03 PM »
In my experience is really not a problem if you passage them at 100%, especially with HEK cells. When you passage them just keep in mind to dilute them a bit more since you've more cells than usual.

Offline WWang1991

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Re: Cell culture maintenance and confluency
« Reply #2 on: March 11, 2015, 03:03:05 AM »
Cell passaging or splitting is a technique that enables an individual to keep cells alive and growing under cultured conditions for extended periods of time. Most of Cells should be passed when they are 90%-100% confluent.
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Offline TYuanyuan

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Re: Cell culture maintenance and confluency
« Reply #3 on: June 15, 2015, 04:45:07 AM »
In my experience is really not a problem if you passage them at 100%, especially with HEK cells. When you passage them just keep in mind to dilute them a bit more since you've more cells than usual.
Agree. it won't be that bad.

Re: Cell culture maintenance and confluency
« Reply #3 on: June 15, 2015, 04:45:07 AM »