General Research Topics > Cell Biology

Cell culture maintenance and confluency

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ic2133:
Hi,

I am growing HEK 293 cells. I know you're supposed to passage them at 70%, but I let them grow to 100% by accident.  Can I still pass them and maintain them, as long as my experiments are done on 70% confluent cells? How bad is it that it got to 100%?

Thank you!

AArwen:
In my experience is really not a problem if you passage them at 100%, especially with HEK cells. When you passage them just keep in mind to dilute them a bit more since you've more cells than usual.

WWang1991:
Cell passaging or splitting is a technique that enables an individual to keep cells alive and growing under cultured conditions for extended periods of time. Most of Cells should be passed when they are 90%-100% confluent.

TYuanyuan:

--- Quote from: AArwen on January 16, 2013, 05:43:03 PM ---In my experience is really not a problem if you passage them at 100%, especially with HEK cells. When you passage them just keep in mind to dilute them a bit more since you've more cells than usual.

--- End quote ---
Agree. it won't be that bad.

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