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Author Topic: cell culture and confluency  (Read 3174 times)

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Offline ic2133

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cell culture and confluency
« on: December 17, 2012, 01:43:37 PM »
Hi,

I am growing HEK 293 cells. I know you're supposed to passage them at 70%, but I let them grow to 100% by accident.  Can I still pass them and maintain them, as long as my experiments are done on 70% confluent cells? How bad is it that it got to 100%?

Thank you!

cell culture and confluency
« on: December 17, 2012, 01:43:37 PM »

Offline Siv

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Re: cell culture and confluency
« Reply #1 on: December 18, 2012, 04:18:10 PM »
From my experience you should be fine.

Re: cell culture and confluency
« Reply #1 on: December 18, 2012, 04:18:10 PM »