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antigen retrieval buffer

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Offline NaNa

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antigen retrieval buffer
« on: January 22, 2013, 07:05:00 AM »
Hi,
can I reuse the antigen retrieval buffer soln once I use it or I have to prepare it every immunostaining?
your help appreciated
Tnx.

antigen retrieval buffer
« on: January 22, 2013, 07:05:00 AM »

Offline gula

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Re: antigen retrieval buffer
« Reply #1 on: January 22, 2013, 12:19:25 PM »
If you use it boiling, you have to make it every time new. Water evaporates, ionstrength varies....
If you use it at temperatures about 70 degrees, I think, you can take it for more times.
gula

Offline NaNa

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Re: antigen retrieval buffer
« Reply #2 on: January 23, 2013, 05:12:40 AM »
Thanks  ;)

Re: antigen retrieval buffer
« Reply #2 on: January 23, 2013, 05:12:40 AM »