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Author Topic: Evans Blue Comtability with Immuno  (Read 2992 times)

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Offline GuitarGuy12

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Evans Blue Comtability with Immuno
« on: January 17, 2013, 12:52:13 PM »
Good afternoon, I have been trying to use Evans blue as an indicator of blood brain barrier permeability.  After a brief circulation I see clear leakage in our mutants but not in the wild type controls, which can be seem by eye and on freshly cut, unfixed frozen sections in the texas red channel. After processing for immuno the Evans blue signal is severely decreased and does not accurately represent the true effect observed.   We have confirmed many of the hypothesized vascular defects by several molecular and ultra structure analysis.   It would really nice to be able to costain these sections with endothelial markers to illustrate the origin of leakage with a bit more specificity... Does anyone know of a way to preserve this signal?    To my knowledge EB is not soluble in acetone so I have been using this as a fixation method.   Likewise, I have noticed that perfusion with PFA flushes out the signal as well.   Any feedback would be sincerely appreciated!

Evans Blue Comtability with Immuno
« on: January 17, 2013, 12:52:13 PM »