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Author Topic: Counting macrophages (F4/80)  (Read 3703 times)

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Offline JHanapi

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Counting macrophages (F4/80)
« on: February 12, 2013, 10:08:07 PM »
Hi guys,

Does anyone know how to count macrophages. I've been told that we can simply count the positive cells but here is the problem

If there is a large positive staining covering a whole single adipocyte, does it count as 1 or 2 or 3 or 4 macrophages? also if there is a small/slight positive staining, is it considered as 1 macrophage.

Hope any of you can help me out. Many thanks

Counting macrophages (F4/80)
« on: February 12, 2013, 10:08:07 PM »

Offline MT Scientist

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Re: Counting macrophages (F4/80)
« Reply #1 on: February 13, 2013, 09:59:49 AM »
I suggest you look for nuclei (the size of the cytoplasm should be irrelevant).
Macrophages will fuse together (Giant cell) and the number of nuclei present should be an indication of numbers.  As to counting, it will depend on your study parameters.  Are giant cells counted as Macs or are they in their own category?

At least this is how I would approach it.  Your milage may vary....

Re: Counting macrophages (F4/80)
« Reply #1 on: February 13, 2013, 09:59:49 AM »