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Author Topic: TUNEL stain for human skeletal muscle  (Read 2100 times)

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Offline VSimon

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TUNEL stain for human skeletal muscle
« on: May 29, 2013, 07:26:57 PM »
Hi guys,

I'm trying to quantify apoptotic nuclei in my human skeletal muscle biopsy specimens which I've formalin fixed and paraffin embedded.

I've been using the Genscript TUNEL kit.

So far only the DNase treated specimen is showing brown nuclear staining. Colon tissue treated with AOM, doesn't show any TUNEL positive cells!

Does anyone have any experience or tips regarding this?

Cheers,

TUNEL stain for human skeletal muscle
« on: May 29, 2013, 07:26:57 PM »