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Author Topic: er beta  (Read 5814 times)

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Offline margarete

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  • Posts: 2
er beta
« on: November 02, 2004, 10:54:01 AM »
I would greatly appreciate any advice (protocol) for ER-beta. :?
Thank you very much
Margaret

er beta
« on: November 02, 2004, 10:54:01 AM »

Offline Spencer

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Re: er beta
« Reply #1 on: January 06, 2010, 04:36:19 PM »
Pa1-01 is the Ab we use.  Manufacturer suggest 1:50 dilution butI've found 1:200 works just as well. 
AR: 10mM sodium citrate pH 6.0 30 min at 96C
Quench: 5% h2o2 in Methanol 30 min
Block: 5% BSA 5% Nonfat milk 5% FBS 1 hour
1 ab 1:200 dilution o/n
2nd 1:500 anti rabbit 1 hour
ABC 30 min
DAB
Counterstain, dehydrate, coverslip done!

Re: er beta
« Reply #1 on: January 06, 2010, 04:36:19 PM »