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Author Topic: Letting FFPE slides dry after deparaffinization  (Read 3338 times)

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Offline Siv

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Letting FFPE slides dry after deparaffinization
« on: July 23, 2013, 04:17:55 PM »
Has anyone observed any adverse effects with letting FFPE tissue dry out after deparaffinization for immuno-staining applications?

Letting FFPE slides dry after deparaffinization
« on: July 23, 2013, 04:17:55 PM »

Offline gula

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Re: Letting FFPE slides dry after deparaffinization
« Reply #1 on: July 24, 2013, 10:25:04 AM »
not personally. But for IHC it was always a No-Go to let them dry after rehydration.
gula

Offline Siv

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Re: Letting FFPE slides dry after deparaffinization
« Reply #2 on: July 25, 2013, 12:30:41 PM »
Hi Gula,

Another sort of related question, have you observed citrate buffer (for antigen retrieval) going bad  (i.e. come out of solution or be light sensitive)?

Offline gula

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Re: Letting FFPE slides dry after deparaffinization
« Reply #3 on: July 25, 2013, 12:54:57 PM »
Sorry, I have no experience with this, but our commercial citric buffer has an exporation date about 6 months. Before we used to buy the buffer, it was mixed fresh every time from two stock solution. But that has been several years ago ....
gula

Re: Letting FFPE slides dry after deparaffinization
« Reply #3 on: July 25, 2013, 12:54:57 PM »