Abcam Ad

Author Topic: Thickness for mouse testis sections  (Read 2777 times)

0 Members and 1 Guest are viewing this topic.

Offline SMancino

  • Newbie
  • *
  • Posts: 1
Thickness for mouse testis sections
« on: August 14, 2013, 11:59:56 AM »
Hi,

I've been playing around with sectioning mouse testes and I found that 5 micron sections work best for light microscopy and H&E staining. This got me wondering why would 5 microns be best.

Have any of you ever wondered why certain thicknesses are fine for certain tissues but not for others?  I would greatly appreciate if anyone could shed some light on this, it's really bugging me.

Thanks a lot,

Steven

Thickness for mouse testis sections
« on: August 14, 2013, 11:59:56 AM »

Offline gula

  • Global Moderator
  • GoldMember
  • *****
  • Posts: 356
Re: Thickness for mouse testis sections
« Reply #1 on: August 15, 2013, 08:58:09 AM »
For diagnostic morphology it is always an aim to get one-cell-layer slides. Therefore the smaller the cells in the tissue and the richment of the cells is a point of decision.
E.g. fatty tissue can be cut at 10 m without troubles in diagnostic, but lymphnodes or bonemarrow must be cut at 1-2 m.
At least you have to "look through" well.
gula

Re: Thickness for mouse testis sections
« Reply #1 on: August 15, 2013, 08:58:09 AM »