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Author Topic: Rough sections  (Read 2183 times)

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Offline SChristina

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Rough sections
« on: October 21, 2013, 09:49:58 AM »
I am sectioning paraffin embedded fat. When I try to section the samples, the fat shreds as soon as it hits the blade. I cannot salvage anything. What is going on?

Rough sections
« on: October 21, 2013, 09:49:58 AM »

Offline gula

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Re: Rough sections
« Reply #1 on: October 22, 2013, 11:28:18 AM »
The fat is not processed properly. The paraffin infiltration wasn't completed and the fat is still contaminated with water or xylen.
Melt the block again and put it over night in melted paraffin. The xylen should solve away and evaporate. then block it out again and try to cut again.
gula

Re: Rough sections
« Reply #1 on: October 22, 2013, 11:28:18 AM »