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Author Topic: Help! ?Bubbles under sections  (Read 2781 times)

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Offline HClarry

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Help! ?Bubbles under sections
« on: November 21, 2013, 09:44:13 AM »
Can anyone advise?
recently we have noticed what look like small bubbles under our ICC stained sections which are noticable under low power as they look like they are staining slightly stronger, and when you go to high power they look like they have lifted.  We first noticed them on tonsil controls with CD20, BCL2 etc which stain strong all over.  They are on both citrate and EDTA treated sections and we use a Thermo Labvision PT Module.  I thought it was because the buffers were boiling but it is set to no-boil.  Does anyone know what causes this?
Thanks,
Helen

Help! ?Bubbles under sections
« on: November 21, 2013, 09:44:13 AM »

Offline iLOVEihc

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Re: Help! ?Bubbles under sections
« Reply #1 on: November 27, 2013, 07:13:47 AM »
Hello, it could be that water has contaminated your xylene, as it has not been removed sufficiently during the alcohol baths.
IHClovER

Re: Help! ?Bubbles under sections
« Reply #1 on: November 27, 2013, 07:13:47 AM »