Author Topic: Staining for rRNA with the Y10B antibody  (Read 2687 times)

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Offline SSpeese

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Staining for rRNA with the Y10B antibody
« on: December 03, 2013, 01:18:07 AM »
I am wondering if anyone has any experience using the Y10B antibody to stain for rRNA.  Supposedly, it recognizes the 5.8S rRNA.  The antibody is a mouse IgG3.  I have not had much luck getting results on whole mount Drosophila tissue that look like the results I have seen from cultured cells.  The main difference is that I get almost no signal from the nucleolous, which should have a high concentration of 5.8S rRNA.  The images from almost every cell culture image I have seen shows high levels of staining in the nucleolous.

Thanks,
   Sean Speese

Research Assistant Professor
OHSU

Staining for rRNA with the Y10B antibody
« on: December 03, 2013, 01:18:07 AM »

Offline AnneHowell

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Re: Staining for rRNA with the Y10B antibody
« Reply #1 on: April 15, 2015, 03:59:37 AM »
I think it may be the quality problem of your antibody, where did you get it?.  It should be no problem to use rRNA Antibody (Y10b) .

Offline JJoanna

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Re: Staining for rRNA with the Y10B antibody
« Reply #2 on: June 27, 2016, 05:59:47 AM »
I think it may be the quality problem of your antibody, where did you get it?.  It should be no problem to use rRNA Antibody (Y10b) .

mouse IgG3 antibody from http://goo.gl/y99sTZ

Re: Staining for rRNA with the Y10B antibody
« Reply #2 on: June 27, 2016, 05:59:47 AM »