I am wondering if anyone has any experience using the Y10B antibody to stain for rRNA. Supposedly, it recognizes the 5.8S rRNA. The antibody is a mouse IgG3. I have not had much luck getting results on whole mount Drosophila tissue that look like the results I have seen from cultured cells. The main difference is that I get almost no signal from the nucleolous, which should have a high concentration of 5.8S rRNA. The images from almost every cell culture image I have seen shows high levels of staining in the nucleolous.
Research Assistant Professor