Author Topic: room temperature antigen retrieval for ICC  (Read 2375 times)

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Offline WWeiwei

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room temperature antigen retrieval for ICC
« on: February 25, 2014, 04:28:21 PM »
Hi,

Anyone has done room temperature  antigen retrieval on ICC. I tried the regular heat induced antigen retrieval, but because I used primary cell and the detachment of the cell to the coated glass slip wan't strong enough, there were no many cell left after the heating.

I found the room temperature protocol says it's for IHC, just want to know if anyone has tried it on cells?

Thanks a lot!

ww

room temperature antigen retrieval for ICC
« on: February 25, 2014, 04:28:21 PM »

Offline richard03

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Re: room temperature antigen retrieval for ICC
« Reply #1 on: March 11, 2014, 03:43:05 PM »
use triton x 100 0.5% should work fine at room temp.

Re: room temperature antigen retrieval for ICC
« Reply #1 on: March 11, 2014, 03:43:05 PM »