Author Topic: Protocol for cFOS and GAD65 IF double labeling in mice?  (Read 1856 times)

0 Members and 1 Guest are viewing this topic.

Offline PrazNina

  • Newbie
  • *
  • Posts: 2
Protocol for cFOS and GAD65 IF double labeling in mice?
« on: February 27, 2014, 04:28:23 AM »
I am looking for a way to do colocalization of GAD65 and fos in neurons in specific brain regions in order to asses which proportion of activated neurons is GABAergic in opposition to glumatergic. I am in search of a promising immunofluorescence protocol, but literature search wasn't successful so far. Does anyone have a paper in mind which describes a protocol how to do this? (which buffer, which antibodies and dilutions...)

I've heard from several sources that it's very difficult to visualize cells stained for GAD65, so also if you have another marker for GABAergic cells which would work better, please share the info!

Thanks!

Protocol for cFOS and GAD65 IF double labeling in mice?
« on: February 27, 2014, 04:28:23 AM »