I am looking for a way to do colocalization of GAD65 and fos in neurons in specific brain regions in order to asses which proportion of activated neurons is GABAergic in opposition to glumatergic. I am in search of a promising immunofluorescence protocol, but literature search wasn't successful so far. Does anyone have a paper in mind which describes a protocol how to do this? (which buffer, which antibodies and dilutions...)
I've heard from several sources that it's very difficult to visualize cells stained for GAD65, so also if you have another marker for GABAergic cells which would work better, please share the info!