Author Topic: Formalin-fixed brains  (Read 2470 times)

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Offline Shariar416

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Formalin-fixed brains
« on: March 03, 2014, 08:08:39 AM »
Hi there,
I have perfused rats' brain with formalin instead of 4% PFA, now I want to do IF on these brains, but it seems that fluorescent secondaries do not works!
Can anyone help me?! the staining is for c-fos.

Thanks,
Shariar

Formalin-fixed brains
« on: March 03, 2014, 08:08:39 AM »

Offline gula

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Re: Formalin-fixed brains
« Reply #1 on: March 03, 2014, 03:06:36 PM »
For IF chemistry there is no difference between neutral buffered formalin and paraformaldehyde. Have you done antigen retrieval? Perhaps another failure causes the bad result.
gula

Re: Formalin-fixed brains
« Reply #1 on: March 03, 2014, 03:06:36 PM »