Methods and Techniques Discussion > Immunofluorescence (IF)

Formalin-fixed brains

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Shariar416:
Hi there,
I have perfused rats' brain with formalin instead of 4% PFA, now I want to do IF on these brains, but it seems that fluorescent secondaries do not works!
Can anyone help me?! the staining is for c-fos.

Thanks,
Shariar

gula:
For IF chemistry there is no difference between neutral buffered formalin and paraformaldehyde. Have you done antigen retrieval? Perhaps another failure causes the bad result.
gula

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