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Author Topic: Antibody Source for c-Fos Immunohistochemistry  (Read 5518 times)

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Offline KGrasing

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Antibody Source for c-Fos Immunohistochemistry
« on: March 04, 2014, 11:30:33 AM »
Hi Histolab,

   Identification of c-Fos immunoreactive cells is a measure neuronal activation.  But there is some variability in results with different antibody sources.   Some earlier posts describe problems with the c-fos antibody from Santa Cruz.  The c-Fos anti-rabbit monoclonal primary antibody from Calbiochem (Cat # PC38) was offered as a solution, but it has since been discontinued.  Milipore which has apparently acquired Calbiochem, is now offering polyclonal anti-c-Fos Antibody raised in rabbits (ABE457, $249 / 100 ug) as a replacement.  Sigma offeres chicken polyclonal anti-c-Fos (GW21144, $258 / 50 ug).  Any ideas or comments?

Thanks,
Ken


Antibody Source for c-Fos Immunohistochemistry
« on: March 04, 2014, 11:30:33 AM »

Offline Histolab

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Re: Antibody Source for c-Fos Immunohistochemistry
« Reply #1 on: January 26, 2015, 09:45:58 AM »
Hi Ken,

 After numerous problems with PC38 from chemicon and SC-52 from santa cruz  tries with Chemicon and santa cruz, we have started using C-fos anti-rabbit polyclonal from a company called Synaptic Systems and so far it has been working very well. Unfortunatly this comes in very small volume of 50ul compared to the previous two and costs a lot more.

regards
Histolab

Offline WWang1991

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Re: Antibody Source for c-Fos Immunohistochemistry
« Reply #2 on: May 07, 2015, 01:55:16 AM »
well, in terms of antibodies sources, you can try to know about the Creative Diagnostics(http://www.creative-diagnostics.com), they can help you out.
The secret to happiness is to keep setting yourself new challenges.
http://blog.creative-bioarray.com/

Re: Antibody Source for c-Fos Immunohistochemistry
« Reply #2 on: May 07, 2015, 01:55:16 AM »