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Author Topic: IF/WB in one brain  (Read 3246 times)

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Offline DLindquist

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IF/WB in one brain
« on: March 28, 2014, 08:05:24 PM »
Hi. If I can, I'd like to do WB in one hemisphere and IF in the other of rat brain. The issue is sacrifice, we use dissection (following decap) for blots and perfusion for IF. Not sure the two can be reconciled. Does anyone know if possible or can suggest a procedure? Thanks.

IF/WB in one brain
« on: March 28, 2014, 08:05:24 PM »

Offline IHCtechMS

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Re: IF/WB in one brain
« Reply #1 on: July 01, 2014, 11:12:31 AM »
Depending on what you are staining for by IF, you could try drop-fixing the half brain for IF. I've used this technique for mouse brains. We typically drop fix overnight.

Re: IF/WB in one brain
« Reply #1 on: July 01, 2014, 11:12:31 AM »