Hi, I would recommend to cover the whole section with your antibody solution in a horizontal position of the slide. The section can be circular marked with a Pap-Pen, in a small distance to the tissue. Incubation has to be done in a moist environment, so use a "wet chamber" for incubation to inhibit drying of the tissue escpecially on the edges. This would lead to unspecific background staining.
Es soll eine regelrechte Flüssigkeitshaube entstehen.
Rinsing of the slide can be done in plenty of buffer in a coplin jar. Use everytime a fresh portion of buffer for rinsing. Move the slide gently in the liquid in order not to loose the tissue.
Der Objektträger mit dem anhaftenden Gewebeschnitt kann in einer mit Puffer gefüllten Küvette sehr einfach gespült werden.
hope this helps
Gula