I'm doing tissue punches from brain for RNA analysis and am running into some problems.
I'm trying to section 300 uM on the cryostat to get good, accurate, tissue punches, but the sections keep rolling. If I try to stop them from rolling then they just crack and break off. I'm trying temperatures as low as -7, and I'm about to try -12. Has anyone had a similar issue, or has experience with this?