Methods and Techniques Discussion > Immunofluorescence (IF)

Autofluorescence in epithelial ovaric cancer


Hi everyone,

i'm having problems of autofluorescence with this tissue :(

The biopsy is fixed with Bouin, the problem is that i just receive the slides done :-\ i'm pretty sure that the bouin is the reason of the autofluorescence.

someone have worked with this fixator in IF before?? can someone help me with a protocol?

Thanks  :)


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