I am trying to do a immunohistochemistry for DRD1 in the Nucleus accumbens (Acb) region. I have tried Abcam (ab78021) and Millipore (MAB5290) with different dilutions ranging from 1:50 - 1:500 for Abcam and 1:100 - 1:500 for millipore. But none seems to work in the Acb and neither in the cortex (+ve control). I do a double immuno with Zif268 which works pretty well.
Therefore, Could you please suggest for DRD1 antibody suggestions that works in the Acb region and general suggestions as well.