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Author Topic: What is the best post fixation time in Perfusion?  (Read 2491 times)

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Offline innsbrucker

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What is the best post fixation time in Perfusion?
« on: December 02, 2014, 12:41:17 PM »
Hello,

I am new to perfusion and have seen many protocols which vary. Recently, i came across a protocol where there was a Post-fixation time in 4% PFA for 8 hours and then transferred to Sucrose whereas other have a post-fixation time from 10 mins to 30 mins and in some cases 1h.

Am i right in saying that the post-fixation time affects the staining too? Please advice.

I am using mice for my experiments and looking at DRD1 and DRD2 neurons in the Accumbens region of the brain.

What is the best post fixation time in Perfusion?
« on: December 02, 2014, 12:41:17 PM »