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Author Topic: Is biotin blocking necessary on human spleen IHC?  (Read 2193 times)

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Offline angela

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Is biotin blocking necessary on human spleen IHC?
« on: January 12, 2015, 11:44:49 AM »
Hi everybody,

I have a question about biotin blocking. I have to do some IHC staining on human spleen paraffin sections. I remember that biotin blocking was quite important when performing IHC on mouse spleen, and I'm wondering how crucial this is in human spleen.

Thank you in advance for your comments,

Angela  :)

Is biotin blocking necessary on human spleen IHC?
« on: January 12, 2015, 11:44:49 AM »

Offline gula

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Re: Is biotin blocking necessary on human spleen IHC?
« Reply #1 on: January 14, 2015, 09:41:39 AM »
yes, you should block the endog. biotin.
gula

Offline immuno

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Re: Is biotin blocking necessary on human spleen IHC?
« Reply #2 on: March 05, 2015, 11:11:39 AM »
Hi:

If you are using a biotin-based method you should block it, if you are using a polymer detection sistem like Envision this step is not necesary.

best

Immuno


Offline SBella

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Re: Is biotin blocking necessary on human spleen IHC?
« Reply #3 on: March 29, 2015, 01:52:12 AM »
agreed immuno. I know biotin is quite important for human,  also called as vitamin H or coenzyme R; Biotin not only to prevents hair loss, it also plays an important role in maintaining the health of the skin. As for the effectiveness of the nervous system stability has not yet been confirmed, but it certainly has some help in the depression, insomnia.

Re: Is biotin blocking necessary on human spleen IHC?
« Reply #3 on: March 29, 2015, 01:52:12 AM »