Author Topic: CYP11A1 IHC-P  (Read 1334 times)

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Offline EBallarini

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CYP11A1 IHC-P
« on: April 21, 2015, 11:58:13 AM »
Can anyone suggest me a good Ab to stain CYP11A1 (cytochrome P45011a1) on mouse tissue?

Good morning.

I have a problem. I'm trying to stain CYP11A1 on Mouse tissues embedded in parrafin or frozen but I can't find a good Ab.

I tryed 3 different Ab on mouse testis and adrenal gland (positive control) embedded in paraffin or frozen in OCT . I changed many protocols (antigen retrival, blocking, Ab concentration, washing buffers, timing, etc etc) I wrote to all techical support and to authors of papers who used the same Ab but I never was able to obtain a good and specific staining (low signal and high background).

These are the Ab I used:

    Abcam cyp11a1 ab 75497
    Santa cruz cyp11a1 (D-16) sc18040
    Proteintech 133663-1-AP

Can you suggest a different Ab to use?

CYP11A1 IHC-P
« on: April 21, 2015, 11:58:13 AM »

Offline WWang1991

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Re: CYP11A1 IHC-P
« Reply #1 on: April 30, 2015, 03:35:49 AM »
maybe this http://www.creative-bioarray.com/can help you out
The secret to happiness is to keep setting yourself new challenges.
http://blog.creative-bioarray.com/

Offline EBallarini

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Re: CYP11A1 IHC-P
« Reply #2 on: April 30, 2015, 04:22:40 AM »
Thank you very much!
I'll try!

Regards
Elisa

Offline YKonstantin

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Re: CYP11A1 IHC-P
« Reply #3 on: July 28, 2016, 05:51:12 AM »
Hello,
I would recommend to search in an antibody database available at www.labome.com. For antibodies to CYP11A1 you may check this link: http://www.labome.com/review/gene/human/DRD1-antibody.html. In addition to the antibodies you have mentioned, Santa Cruz also has goat polyclonal (C-16) and rabbit polyclonal (H-165) antibodies suitable to detect CYP11A1 on mouse tissues. Also, OriGene has a rabbit polyclonal antibody to CYP11A1 suitable for IHC. In addition, there are several rabbit polyclonal antibody to CYP11A1, provided by Invitrogen (PA5-37359), MyBioSource (MBS128059) and US Biological (210368), which can be used for IHC on mouse tissues.

Offline MMaggie

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Re: CYP11A1 IHC-P
« Reply #4 on: October 03, 2016, 05:26:37 PM »
thanks for the mention of CYP11A1 - (MBS128059) if you have any questions or concerns let me know and I'll be happy to answer them.

"Our primary mission at MyBiosource, Inc. is to provide #1 quality reagents worldwide."

Re: CYP11A1 IHC-P
« Reply #4 on: October 03, 2016, 05:26:37 PM »