Can anyone suggest me a good Ab to stain CYP11A1 (cytochrome P45011a1) on mouse tissue?
I have a problem. I'm trying to stain CYP11A1 on Mouse tissues embedded in parrafin or frozen but I can't find a good Ab.
I tryed 3 different Ab on mouse testis and adrenal gland (positive control) embedded in paraffin or frozen in OCT . I changed many protocols (antigen retrival, blocking, Ab concentration, washing buffers, timing, etc etc) I wrote to all techical support and to authors of papers who used the same Ab but I never was able to obtain a good and specific staining (low signal and high background).
These are the Ab I used:
Abcam cyp11a1 ab 75497
Santa cruz cyp11a1 (D-16) sc18040
Can you suggest a different Ab to use?