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Author Topic: HLA-DQ antibody for paraffin sections  (Read 6226 times)

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Offline MMG

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HLA-DQ antibody for paraffin sections
« on: October 01, 2004, 02:50:19 PM »
I am trying to get a HLA-DQ antibody (clone SPV-L3) to work on paraffin sections.  Does anyone have any suggestion, insights, advice?  Thanks so much.

MMG

HLA-DQ antibody for paraffin sections
« on: October 01, 2004, 02:50:19 PM »

Offline richard03

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HLA-DQ antibody for paraffin sections
« Reply #1 on: October 01, 2004, 04:09:32 PM »
You may find this paper helpful.

Characterization of anti-HLA class II monoclonal antibody LGII-612.14 reacting with formalin fixed tissues
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8505553&dopt=Abstract

Richard

Offline MMG

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HLA-DQ antibody for paraffin sections
« Reply #2 on: October 01, 2004, 04:21:35 PM »
Richard-
Thanks for the reference.  Unfortunately that is a pan-HLA Class II antibody that detects DR, DQ and DP.  I need one that is DQ specific.

Megan

HLA-DQ antibody for paraffin sections
« Reply #2 on: October 01, 2004, 04:21:35 PM »