Author Topic: Length of time to section Adult Rat Brain  (Read 1271 times)

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Offline MJennifer

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Length of time to section Adult Rat Brain
« on: July 23, 2015, 01:41:08 PM »
Hello,

I would like to know the average time it takes an experienced histologist to make 10 micron cryosections of a 4% PFA fixed adult rat brain (excluding the cerebellum).  It currently takes myself and our medical student 4-5 hours to section and immediately place 3-4 sections on each superfrost positive charged slide.  My mentor wants us to be more efficient but I was curious to know if our timeframe is similar to others.

Thank you!

Jennifer

Length of time to section Adult Rat Brain
« on: July 23, 2015, 01:41:08 PM »

Offline gula

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Re: Length of time to section Adult Rat Brain
« Reply #1 on: July 25, 2015, 04:39:48 AM »
Hi Jennifer,
what does it make so difficult to cut the brains? Do you speak only about cutting or also about specimen-preparation before cutting and staining afterwards?

In clinical histology we do fast frozens. The tissue (15x15x5 mm) takes about 2-3 min to be frozen, the cutting is done for 2 sections in 1-2 min, then staining.... The average time from income of the specimen in the lab until ready report to the surgery is about 18-20 min.
gula


Re: Length of time to section Adult Rat Brain
« Reply #1 on: July 25, 2015, 04:39:48 AM »