Methods and Techniques Discussion > Immunofluorescence (IF)

How two use two primary raised in same species in IFC


Is there any way to use two primary Antibodies raised in same species.
My both primary are raised in Rabbit. Your expert help will be appreciated.

I have the same issue right now as well. First, I tried looking for another primary I could swap out, but unfortunately I had no luck. My next thought is to possibly incubate the primary antibodies with secondaries before IFC so that I create primaries with secondaries already attached. I could also purchase this already made (my target primary with an Alexa Fluor conjugate attached), but I have no previous experience with this and don't know how well it would work when primaries will have matching hosts. I would love to hear if anyone else has tried this or what other techs did to get around this.


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