Author Topic: Manual staining of human liver tissue with Dako REAL™ EnVision™ Detection System  (Read 612 times)

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Offline DAlmuaili

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Hello,
I'm manually staining paraffin embedded human liver tissue for CD3 using the DAKO kit. My protocol works great when staining 4 slides or less. however it stops working on any more than 5 slides. Any ideas on what may be the reason? is it maybe the slides are drying out before I am ready to load them up with the next solution?
Thanks!