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Author Topic: Embedding large tissue samples for thick section cutting at RT  (Read 2179 times)

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Offline AAnneke

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I am looking for an embedding medium which will allow me to process very large brain tissue samples at room temperature. The tissue sample does not fit in any cryostate available within a reasonable distance, so freezing is not an option. I need thicker sections (50-100) micrometer, so paraffin it not a good option either, and since removing the tissue from the microtome will interfere with the research goal, celloidin is not realistic. Can anyone suggest a different embedding medium?