Author Topic: Ethanol fixed samples for TEM?  (Read 674 times)

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Offline Sieve

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Ethanol fixed samples for TEM?
« on: July 12, 2016, 12:34:58 PM »
Hello,

I have recently started doing a lot of TEM work and came across samples (cells grown on an air liquid interface) from a year or so back fixed with ethanol (and are currently stored in 100% ethanol).

They were never fixed for TEM and have only been exposed to ethanol. I know the general protocol calls for glut/formaldehyde fixation, but what happens to samples fixed with ethanol/methanol that are then processed for EM?

Thanks
« Last Edit: July 14, 2016, 08:29:25 AM by Sieve »

Ethanol fixed samples for TEM?
« on: July 12, 2016, 12:34:58 PM »