Methods and Techniques Discussion > Immunofluorescence (IF)

Using the dark room for IF staining

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AAdrienne:
Hello,

I'm brand new to IF and I'm kind of flying solo in my lab, so this will be a very amateur question! I'm about to stain with the fluorochrome secondary antibody - obviously I need to do this in a dark room....but how dark does it need to be? How can I make up my antibody in the dark? I'd really appreciate the help - thanks!

gula:
not really dark. One should avoid sunlight because of UV light. Keep the reagens tubes in a drawer or in light-dense tubes. Cover the sections in a humid chamber with something light-dense (bigger box,  dark Cloth).

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