This is probably a too elementary question and it is indeed, I don' have a lot of experience with histology. We have a microtome in our lab and we always used frozen sections. However, I need thinner slices for the analysis that I am trying to make (HE on adrenal glands), and can't get good sections of around 10um using frozen tissue. 30um is the thinnest with good quality.
I started inquiring about changing to paraffin embedded but we don't have the equipment. I might be missing something, but someone mentioned frozen paraffin sections. Does anyone know how it is done? Assuming paraffin is already solid on RT, how could you place it securely on a microtome that cuts frozen sections?
Also for tissue preparation, most of my samples have already been collected and are in sucrose solution. Is it okay to just change medium, or since they have been sitting in sucrose for a while now, best way to go is to continue with frozen protocol?
Thank you for your help