Author Topic: Staining on only the periphery of tissue  (Read 1016 times)

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Offline AAdrienne

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Staining on only the periphery of tissue
« on: September 01, 2016, 04:29:49 PM »
I've been working on some FISH/IHC combo stains which have been working well until this last run where I got bright staining on the edge of the tissues but very minimal staining in the middle. The only difference this time was that I used PFA that I had made last week, aliquoted and frozen. I did some reading up on freezing PFA and I know there are mixed reviews. Does anyone have insight on what might have happened? I purchase the 16% PFA ampules so I don't mind making it up fresh every time; I was just trying to save the solution so I don't have to buy it all the time. I don't know what else it could have been. Thanks in advance for any tips!

Staining on only the periphery of tissue
« on: September 01, 2016, 04:29:49 PM »

Offline QChong

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Re: Staining on only the periphery of tissue
« Reply #1 on: February 13, 2017, 05:06:52 PM »
someone suggest me to cover the antibody drop with a parafilm to avoid edge effect. may be you can try. good luck.

Re: Staining on only the periphery of tissue
« Reply #1 on: February 13, 2017, 05:06:52 PM »