Hello all: a lot of info on Ki-67 antibodies, a lot of confusion. Does anyone know whether antigen retrieval is required for formaldehyde-fixed tissue that has NOT been paraffin embedded (i.e., frozen sectioned after graded sucrose)? I would prefer not to have to denature the native fluorescent reporter proteins that are in this tissue.
On the similar topic, any antibody suggestions would also be welcomed. Thank you.