Author Topic: IHC artifact - aspecific staining in some tissue parts  (Read 201 times)

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Offline BCaroline

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IHC artifact - aspecific staining in some tissue parts
« on: January 19, 2017, 10:26:35 AM »
Dear all,

We are facing an issue with chromogenic immunostainings on liver sections and hope you can provide some help.

We observe aspecific immunostaining in tissue regions unrelated to a particular structure (example: http://dih.irec.ucl.ac.be/dih/webViewer.php?snapshotId=14848387200379).
These stainings worked in our hands in the past and we are a bit puzzled by these results...

Those artifacts:
- are not reproducible (other regions aspecifically stained on serial sections stained different days),
- do not seem to be related to primary or secondary antibodies (same pattern for different antibodies - mouse and rabbit),
- do not seem to be caused by altered solutions (same result if using all fresh solutions).

Any advice welcome  :) :)

Thank you in advance,
Caroline




IHC artifact - aspecific staining in some tissue parts
« on: January 19, 2017, 10:26:35 AM »