We are facing an issue with chromogenic immunostainings on liver sections and hope you can provide some help.
We observe aspecific immunostaining in tissue regions unrelated to a particular structure
These stainings worked in our hands in the past and we are a bit puzzled by these results...
- are not reproducible (other regions aspecifically stained on serial sections stained different days),
- do not seem to be related to primary or secondary antibodies (same pattern for different antibodies - mouse and rabbit),
- do not seem to be caused by altered solutions (same result if using all fresh solutions).
Any advice welcome
Thank you in advance,