Author Topic: Cryoprotection-- phosphate buffer saline vs. phosphate buffer  (Read 434 times)

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Offline Aisabella

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Cryoprotection-- phosphate buffer saline vs. phosphate buffer
« on: January 24, 2017, 12:27:33 PM »
Our cryoprotectant recipe calls for 50% phosphate buffer, 30% ethylene glycol, and 20% glycerol. We accidentally used phosphate buffer saline (with the ethylene glycol and glycerol) instead. I have three questions:
1) Will the NaCl interfere with cryoprotection (i.e., will it allow freezing damage to the tissue)?
2) What would freezing damage look like?
and 3) What problems could this mixup cause later with IHC?

Further details: we store our tissue at -20C and want to stain for Ki67 and doublecortin.
Thank you!

Cryoprotection-- phosphate buffer saline vs. phosphate buffer
« on: January 24, 2017, 12:27:33 PM »