I am not sure what else I can do.
The tissue is immersion-fixed in 4% pfa with a pH of 7.4 for 24 hours after perfusion.
After mounting tissue onto poly l lysine slides, I fume fix with 16% for multiple nights and then let dry for a whole day
When running CV, my tissue falls off in the very first few wells: the dH2O and 70% ethanol.
I'm at a loss as everything I find comes down to fixation quality and using coated slides.
Any other tricks out there???