Methods and Techniques Discussion > Immunofluorescence (IF)

unmask by proteinaseK


liver staining by CD31- rat anti mouse antibody(Abcam Catalog Number: ab28364), only the edge and around of the vessels of the liver can be stained clearly(see the attachment).
the liver to be fixed are no more than 3mm, fixed by zink fixation buffer for 72h,
proteinaseK is 20ug/ml, 30min@RT, followed by Permeabilization. 
Could you please help me to find how to stain the tissue evenly? thanks.


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