Methods and Techniques Discussion > Immunofluorescence (IF)

Tdtomato fluorescence disappears in OCT frozen sections

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PEpstein:
I have many frozen tissue tissue blocks containing a cell specific Tdtomato marker.  If the sample was formalin fixed sections retain great tdtomato flourescence. But for samples not formalin fixed sections lose tdtomato flourescence immediately on exposure to any aqueous buffer.  Does anyone know why this happens and how we can prevent loss of tdtomato fluorescence in our OCT non-formalin fixed samples?

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