Author Topic: tissue handling post cutting  (Read 71 times)

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Offline JHofmeister

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tissue handling post cutting
« on: October 16, 2017, 10:23:22 AM »
Half rat brain tissue pfa fixed, frozen cut on cryostat 30microns stored in cryoprotectant.
Brain curled tight while stored and tears easily when trying to uncurl for mounting on gel slides after staining.
any suggestions would be helpful.

tissue handling post cutting
« on: October 16, 2017, 10:23:22 AM »