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Author Topic: Unspecific staining in skin  (Read 972 times)

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Offline DValerie

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Unspecific staining in skin
« on: February 23, 2018, 01:45:07 PM »
Hi!

I am doing IHC in paraffin-embedded tissues and I have problems with a unspecific staining. I work with equine wounds and I know that unspecific staining has been seen in sebaceous glands, but I have one also in the blood clot. I made a test to check if this was probably caused by the endogen avidin/biotin and I got a positive staining even without primary and secondary antibodies (I use ABC with alkaline phosphatase). So, do you guys think I should block endogen avidin/biotin or this may due to something else?

Thank you!

« Last Edit: February 23, 2018, 04:49:59 PM by DValerie »

Unspecific staining in skin
« on: February 23, 2018, 01:45:07 PM »